Elsevier

Kidney International

Volume 49, Issue 5, May 1996, Pages 1342-1349
Kidney International

Laboratory Investigation
Acute passive anti-glomerular basement membrane nephritis in P-selectin-deficient mice

https://doi.org/10.1038/ki.1996.190Get rights and content
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Acute passive anti-glomerular basement membrane nephritis in P-selectin-deficient mice. P-selectin present on surfaces of activated endothelium and platelets mediates neutrophil-endothelial and neutrophil-platelet interactions. The role of P-selectin in vivo was examined in a model of acute passive anti-GBM nephritis in P-selectin-deficient and wild-type mice which was induced by intravenous injection of anti-GBM serum. There were two major differences between P-selectin-deficient and wild-type mice. Firstly, mutant mice had approximately two fold more glomerular PMNs and albuminuria than wild-type animals at the peak of neutrophil influx and proteinuria. Secondly, Lipoxin A4 (LXA4), an eicosanoid which inhibits leukocyte-endothelial adhesion in vitro, and is generated primarily by transcellular biosynthetic routes during P-selectin-mediated platelet-PMN interaction [1], was approximately 60% of wild type levels in nephritic kidneys of P-selectin-deficient mice. Injection of wild-type platelets into P-selectin-null mice restored LXA4 to wild-type levels. The corresponding PMN influx approximated PMN levels in wild-type mice receiving platelets but urine albuminuria remained higher. Although these two P-selectin-dependent events cannot be directly linked, our results point to the importance of considering both platelet and endothelial P-selectin in determining the cellular events in inflammation.

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