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Cloning and expression of the mouse pseudoautosomal steroid sulphatase gene (Sts)

Nature Genetics volume 13, pages 83–86 (1996)Cite this article

Abstract

Steroid sulphatase (STS) Is an important enzyme in steroid metabolism1. The human STS gene has been cloned and mapped to Xp22.3, proximal to the pseudoautosomal region (PAR)2. Using quantitative differences in STS activity among various mouse strains, a segregation pattern consistent with autosomal linkage was first reported3–5, but more recent studies have linked Ste to the mouse PAR6–11. Failed attempts to clone the mouse Sfs gene using human reagents (STS cDNA and anti-STS antibodies) suggest a substantial divergence between these genes. However, partial amino-terminal sequence from purified rat liver Sts is very similar to its human counterpart12, and several domains are conserved among all the sulphatases13–14. We followed a degenerate-primer reverse transcriptase-PCR (RT-PCR) approach to amplify a conserved fragment of the rat Ste cDNA that was then used to clone the mouse Ste cDNA. This 2.3-kb cDNA revealed 75% similarity with rat Sfs cDNA, while it was only 63% similar to human STS cDNA. Transfection of STS(−) A9 cells with the mouse Sfs cDNA restored STS enzymatic activity. Sts was also mapped physically to the distal end of the mouse sex chromosomes, and our backcross studies placed Sts distal to the ‘obligatory’ crossover in male meiosis.

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Author notes

  1. Thuluvancheri K. Mohandas

    Present address: Department of Pathology, Darmouth Medical School, Hanover, New Hampshire, 03755, USA

Authors and Affiliations

  1. Department of Pediatrics, UCSF School of Medicine, Box 0110, 531 Parnassus Ave., San Francisco, California, 94143, USA

    Eduardo C. Salido, Xiao M. Li & Larry J. Shapiro

  2. Division of Medical Genetics, Harbor-UCLA Medical Center, Torrance, California, 90509, USA

    Pauline H. Yen & Thuluvancheri K. Mohandas

  3. Department of Pathology, Universidad La Laguna, E-38071, Tenerife, Spain

    Eduardo C. Salido & Natalia Martin

Authors
  1. Eduardo C. Salido
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  6. Larry J. Shapiro
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Correspondence to Larry J. Shapiro.

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Salido, E., Li, X., Yen, P. et al. Cloning and expression of the mouse pseudoautosomal steroid sulphatase gene (Sts). Nat Genet 13, 83–86 (1996). https://doi.org/10.1038/ng0596-83

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