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Regulation of fast-spiking basket cell synapses by the chloride channel ClC-2

Abstract

Parvalbumin-expressing, fast-spiking basket cells are important for the generation of synchronous, rhythmic population activities in the hippocampus. We found that GABAA receptor–mediated synaptic inputs from murine parvalbumin-expressing basket cells were selectively modulated by the membrane voltage– and intracellular chloride–dependent chloride channel ClC-2. Our data reveal a previously unknown cell type–specific regulation of intracellular chloride homeostasis in the perisomatic region of hippocampal pyramidal neurons.

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Figure 1: Outward rectification of PV-IPSCs and distribution of PVBC axon terminals on the somata of pyramidal cells.
Figure 2: Somatic hyperpolarization-gated, sustained Cl conductance mediated by ClC-2.

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Acknowledgements

We thank R. Zhu for excellent technical assistance, K. Ding and D. Szabadics for camera lucida reconstructions, M. Case for genotyping, J.E. Melvin for the ClC-2 knockout mouse and L. Hilgenberg, M.A. Smith, M. Cahalan, M.V. Jones, Z. Nusser and R.L. McDonald for discussions. This work was supported by US National Institutes of Health grant NS38580 (to I.S.) and the Epilepsy Foundation through the generous support of the Eric W. Lothman Training Fellowship (to S.-H.L.).

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C.F. and S.-H.L. designed the experiments. C.F., S.-H.L. and R.J.M. performed the experiments and analyzed the data. C.F. and I.S. wrote the manuscript.

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Correspondence to Csaba Földy.

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The authors declare no competing financial interests.

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Supplementary Figure 1 and Supplementary Methods (PDF 775 kb)

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Földy, C., Lee, SH., Morgan, R. et al. Regulation of fast-spiking basket cell synapses by the chloride channel ClC-2. Nat Neurosci 13, 1047–1049 (2010). https://doi.org/10.1038/nn.2609

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