Abstract
The last 10 years have seen substantial progress in the development and application of nonviral vectors in gene therapy. However, many problems remain to be resolved before nonviral gene therapy can become a standard clinical practice. This review highlights the major breakthroughs in this field. The problems and future research directions are also discussed.
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References
Wolff JA et al. Direct gene transfer into mouse muscle in vivo Science 1990 247: 1465–1468
Ulmer JB et al. Heterologous protection against influenza by injection of DNA encoding a viral protein Science 1993 259: 1745–1749
Condon C et al. DNA-based immunization by in vivo transfection of dendritic cells Nature Med 1996 2: 1122–1128
Liu F, Song Y, Liu D . Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA Gene Therapy 1999 6: 1258–1266
Yang NS et al. In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment Proc Natl Acad Sci USA 1990 87: 9568–9572
Rols MP et al. In vivo electrically mediated protein and gene transfer in murine melanoma Nat Biotechnol 1998 16: 168–171
Rizzuto G et al. Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation Proc Natl Acad Sci USA 1999 96: 6417–6422
Anwer K et al. Systemic effect of human growth hormone after intramuscular injection of a single dose of a muscle-specific gene medicine Hum Gene Ther 1998 9: 659–670
Felgner PL et al. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure Proc Natl Acad Sci USA 1987 84: 7413–7417
Wheeler CJ et al. A novel cationic lipid greatly enhances plasmid DNA delivery and expression in mouse lung Proc Natl Acad Sci USA 1996 93: 11454–11459
Lee ER et al. Detailed analysis of structures and formulations of cationic lipids for efficient gene transfer to the lung Hum Gene Ther 1996 7: 1701–1717
Nabel GJ et al. Direct gene transfer with DNA–liposome complexes in melanoma: expression, biologic activity, and lack of toxicity in humans Proc Natl Acad Sci USA 1993 90: 11307–11311
Caplen NJ et al. Liposome-mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis Nat Med 1995 1: 39–46
Radler JO, Koltover I, Salditt T, Safinya CR . Structure of DNA–cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes Science 1997 275: 810–814
Subramanian A, Ranganathan P, Diamond SL . Nuclear targeting peptide scaffolds for lipofection of nondividing mammalian cells Nat Biotechnol 1999 17: 873–877
Li S et al. Dynamic changes in the characteristics of cationic lipidic vectors after exposure to mouse serum: implications for intravenous lipofection Gene Therapy 1999 6: 585–594
Gao X, Huang L . Potentiation of cationic liposome-mediated gene delivery by polycations Biochemistry 1996 35: 1027–1036
Liu Y et al. Factors influencing the efficiency of cationic liposome-mediated intravenous gene delivery Nat Biotechnol 1997 15: 167–173
Boussif O et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine Proc Natl Acad Sci USA 1995 92: 7297–7301
Roy K, Mao HQ, Huang SK, Leong KW . Oral gene delivery with chitosan–DNA nanoparticles generates immunologic protection in a murine model of peanut allergy Nat Med 1999 5: 387–391
Cole-Strauss et al. Correction of the mutation responsible for sickle cell anemia by an RNA–DNA oligonucleotide Science 1996 273: 1386–1389
Kren BT et al. Correction of the UDP-glucuronosyltransferase gene defect in the Gunn rat model of Crigler–Najjar syndrome type I with a chimeric oligonucleotide Proc Natl Acad Sci USA 1999 96: 10349–10354
Yew NS et al. Contribution of plasmid DNA to inflammation in the lung after administration of cationic lipid:pDNA complexes Hum Gene Ther 1999 10: 223–234
Li S et al. Effect of immune response on gene transfer to the lung via systemic administration of cationic lipidic vectors Am J Physiol 1999 276: L796–L804
Krieg AM et al. CpG motifs in bacterial DNA trigger direct B-cell activation Nature 1995 374: 546–549
Chu RS et al. CpG oligodeoxy-nucleotides act as adjuvants that switch on T helper 1 (Th1) immunity J Exp Med 1997 186: 1623–1631
Acknowledgements
The original work in this laboratory was supported by NIH grants CA 59327, DK 44935, CA 71731 and a contract from Targeted Genetics Corporation.
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Li, S., Huang, L. Nonviral gene therapy: promises and challenges. Gene Ther 7, 31–34 (2000). https://doi.org/10.1038/sj.gt.3301110
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DOI: https://doi.org/10.1038/sj.gt.3301110
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