Abstract
OBJECTIVE: To investigate whether Trp64Arg polymorphism of the β3-adrenergic receptor (β3-AR) gene and Gln223Arg polymorphism of the leptin receptor (Ob-R) gene are associated with obesity in Japanese schoolchildren.
DESIGN: Population study of participants from a rural town located within 50 km northeast of Tokyo based on school medical examinations.
SUBJECTS: 553 Japanese schoolchildren (291 boys and 262 girls) who were 9–15 y old with a mean age of 11.9±1.8 y.
MEASUREMENTS: DNA was extracted from whole blood and genotyped by PCR-RFLP. Height, weight and blood pressure were measured in school medical examinations. Total cholesterol, triglyceride and HDL-cholesterol concentrations were measured by an autoanalyzer. Obesity index, body mass index (BMI) and LDL-cholesterol concentration were calculated by the respective formulae.
RESULTS: In Trp64Arg polymorphism of the β3-AR gene, the number of obese subjects with Trp/Arg or Arg/Arg genotypes was significantly higher than that of the non-obese subjects (χ2=5.79, P=0.02). The obesity index of subjects with the Arg/Arg or Arg/Trp genotype was significantly higher than that of those with the Trp/Trp genotype (8.2±18.7% vs 4.5±15.8%, P=0.04). Moreover, after adjustments for age and gender, BMI of subjects with the Trp/Arg or Arg/Arg genotype was significantly higher than that of those with the Trp/Trp genotype (19.4±3.6 kg/m2 vs 18.9±3.2 kg/m2, P=0.02). However, no significant differences were observed in the clinical characteristics among the genotype groups of the Ob-R gene.
CONCLUSIONS: Trp64Arg polymorphism of the β3-AR gene appears to be a genetic risk factor for obesity in Japanese children, but Gln223Arg polymorphism of the Ob-R gene does not appear to be associated with obesity.
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This work was supported by a grant for scientific research from the Ministry of Education (09772047, 11770191).
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Endo, K., Yanagi, H., Hirano, C. et al. Association of Trp64Arg polymorphism of the β3-adrenergic receptor gene and no association of Gln223Arg polymorphism of the leptin receptor gene in Japanese schoolchildren with obesity. Int J Obes 24, 443–449 (2000). https://doi.org/10.1038/sj.ijo.0801177
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DOI: https://doi.org/10.1038/sj.ijo.0801177
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