Issue 8, 2010
From the journal:

Molecular BioSystems

A chemical-genetic approach for precise spatio-temporal control of cellular signaling†

Shuyun Dong,a   John A. Allen,a   Martilias Farrella  and  Bryan L. Roth*a  

* Corresponding authors

a Departments of Pharmacology, University of North Carolina Medical School, 4009 Genetic Medicine Building, Chapel Hill
E-mail: bryan_roth@med.unc.edu
Fax: 919-843-5788
Tel: 919-966-7535

Abstract

Recently we have perfected a chemical-genetic approach to gain precise spatio-temporal control of cellular signaling. This approach entails the cell-type specific expression of mutant G-protein coupled receptors which have been evolved to be activated by the pharmacologically inert drug-like small molecule clozapine N-oxide. We have named these mutant GPCRs DREADDs (Designer Receptors Exclusively Activated by Designer Drugs). In this paper we will first review recent applications of this technology for the remote control of neuronal and non-neuronal signaling. Next, we will also introduce new variants which could be useful for the control of cellular signaling in discrete cellular compartments. Finally, we will suggest future basic science and therapeutic applications of this general technology.

Graphical abstract: A chemical-genetic approach for precise spatio-temporal control of cellular signaling

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Article information

DOI
https://doi.org/10.1039/C002568M
Article type
Review Article
Submitted
05 Feb 2010
Accepted
23 Apr 2010
First published
07 Jun 2010

Mol. BioSyst., 2010,6, 1376-1380

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A chemical-genetic approach for precise spatio-temporal control of cellular signaling

S. Dong, J. A. Allen, M. Farrell and B. L. Roth, Mol. BioSyst., 2010, 6, 1376 DOI: 10.1039/C002568M

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