Rapid communicationDes-acyl Ghrelin Acts by CRF Type 2 Receptors to Disrupt Fasted Stomach Motility in Conscious Rats
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Animals
Male Wistar rats (Clea Japan, Tokyo, Japan) weighing 230–280 g at the start of the experiment were used and housed in group cages under controlled illumination (light cycle, 8 am to 8 pm), humidity, and temperature (22.5°C ± 1.5°C) with free access to water and laboratory chow pellets (CE-2; Clea Japan). All experiments were performed between 9 am and 6 pm in fasted, freely moving conscious rats, in accordance with the Guide for Use of Experimental Animals of Shiga University of Medical
Effects of IP Injection of Des-acyl Ghrelin and Acylated Ghrelin on Food Intake in Food-Deprived and Free-Feeding Rats
The effects of des-acyl ghrelin and acylated ghrelin on food intake were examined in food-deprived and free-feeding rats. In 16-hour food-deprived rats, des-acyl ghrelin injected IP induced a significant decrease of 1-hour (4.15 ± 0.37 g) and 2-hour (4.42 ± 0.37 g) cumulative food intake compared with saline-injected controls (5.35 ± 0.30 g and 5.73 ± 0.32 g, respectively) (Figure 1A). On the other hand, acylated ghrelin injected IP induced a significant increase of 1-hour (6.25 ± 0.23 g) and
Discussion
In the present study, we first report the effects of des-acyl ghrelin on food intake and gastroduodenal motility in conscious rat models, and specific brain nuclei responding to these effects were examined compared with acylated ghrelin. In the food intake study, des-acyl ghrelin injected IP effectively decreased 1-hour and 2-hour cumulative food intake in food-deprived rats and also decreased the dark-phase food intake in free-feeding rats but failed to decrease the light-phase food intake in
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Supported by a Grant-in-Aid (14571193) for Scientific Research from the Ministry of Education, Culture, Sports and Technology, Japan (to M.F.).