Original Article
Th2 Cytokine mRNA Expression in Primary Cutaneous CD30-Positive Lymphoproliferative Disorders: Successful Treatment With Recombinant Interferon-γ

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Primary cutaneous CD30 (Ki-1)+ large cell lymphoma (KiL) and lymphomatoid papulosis (LyP) type A are collectively termed as primary cutaneous CD30-positive lymphoproliferative disorders. We examined the cytokine profile of skin-infiltrating cells and the therapeutic efficacy of recombinant interferon-γ (rIFN-γ) in primary cutaneous KiL and LyP type A. By reverse transcriptase-polymerase chain reaction, mRNAs for interleukin-4 (IL-4) and IL-b were detected in the dermis of skin lesions in all cases (three cases of KiL and four cases of LyP). In addition, tissue from one KiL patient transcribed IL-2 and IFN-γ messages, and one LyP patient showed IL-2 mRNA. In contrast, normal skin from ten healthy donors contained mRNA for IL-2 or IFN-γ, or both, but not for IL-4. Before the therapeutic trial of rIFN-γ, the response of skin lesions was assessed by a predictive skin test with local injection of rIFN-γ (0.5 × 106 Japan Reference Units [JRU; 1 JRU roughly corresponds to 4 NIH units]) for 3 consecutive days in two KiL and two LyP patients. Numbers of skin-infiltrating CD30+ cells were decreased, and transcription of mRNA for IL-4 and IL-10 was downregulated after the skin test in one KiL and two LyP cases. One kiL patient showed no histologic response or change in mRNA expression. In the therapeutic trial, rIFN-y (total doses of 1.2-4.0 × 107 JRU) was administered intravenously (n = 2) or locally (n = 2). In three patients who responded to the skin test, the lesions were objectively improved and the numbers of skin-infiltrating CD30+ cells were markedly decreased after the therapeutic trial. No improvement was observed in one KiL patient who did not respond to the skin test. These findings suggest that the skin-infiltrating CD30+ cells in KiL and LyP have a Th2 cytokine profile and raise the possibility that the administration of rIFN-γ improves the conditions by inhibiting cytokine mRNA transcription and proliferation of CD30+ cells.

Keywords

Ki-1+ lymphoma
lymphomatoid papulosis
cutaneous T-ceIl lymphoma
anaplastic large cell lymphoma

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