Chest
Laboratory and Animal InvestigationsAssociation of p53 Gene Mutation and Telomerase Activity in Resectable Non-Small Cell Lung Cancer
Section snippets
Materials and Methods
Forty specimens were sampled from 40 surgical cases of NSCLC between May 1997 and May 1999 (squamous cell carcinoma[n = 21], adenocarcinoma [n = 17], large cell carcinoma[n = 2]). These samples were histologically confirmed to be a part of tumor masses. Samples were frozen in liquid nitrogen immediately after surgical resection and stored at − 80°C until use.
Results
Mutations of the p53 gene were confirmed in 19 of 40 specimens (47.5%), 15 missense mutations, and 4 frameshift mutations. One case, in which the mutation did not cause the alteration of the amino acid, was excluded. The TPG of 40 specimens was 75.24 ± 15.55 (mean ± SE; Tables 1 and 2). TPG of the 19 specimens positive for p53 gene mutation was significantly higher than that of the 21 specimens negative for p53 gene mutation (Fig 2, left, a). The examination of each histologic type showed the
Discussion
The p53 tumor suppressor gene plays two important roles in genomic stability: blocking cell proliferation after DNA damage until it has been repaired,14 and starting apoptosis if the damage is too extensive.15 p53 mutations inactivate the tumor suppressor function of wild-type p53, elevating tumor incidences,16 and mutations are most frequently detected in human cancer.1718 Activation of telomerase extends the telomere length and makes repeated cell divisions possible.91920 Elevation of
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