Abstract
Benzoate X receptor (BXR) is a member of the nuclear hormone receptor superfamily and is activated by alkyl esters of amino benzoic acids and expressed during early development stages. We report here a second BXR, which was cloned by screening Xenopus laevis embryonic cDNA libraries. This new BXR, termed BXRbeta, exhibits 97% identity in the DNA-binding domain and 79% identity in the ligand-binding domain in amino acid sequence to previously reported Xenopus BXR. The BXRbeta strongly binds as a heterodimer with retinoid X receptor (RXR) to direct repeats of an AGGTCA motif spaced 4 or 5 base pairs apart and activates transcription by addition of methyprednisolone and dexamethasone as well as amino- and hydroxybenzoates.
Copyright 2000 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA / genetics
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DNA / metabolism
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Dexamethasone / pharmacology
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Dimerization
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Hydroxybenzoates / pharmacology
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Ligands
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Methylprednisolone / pharmacology
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Molecular Sequence Data
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Protein Binding
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Protein Structure, Tertiary
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Receptors, Cytoplasmic and Nuclear / chemistry*
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Receptors, Cytoplasmic and Nuclear / genetics*
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Receptors, Cytoplasmic and Nuclear / metabolism*
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Receptors, Retinoic Acid / metabolism
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Response Elements / genetics
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Retinoid X Receptors
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Sequence Alignment
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Sequence Analysis, DNA
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Transcription Factors / metabolism
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Transcriptional Activation / drug effects
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Transcriptional Activation / genetics
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Xenopus Proteins*
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Xenopus laevis / embryology
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Xenopus laevis / genetics*
Substances
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DNA-Binding Proteins
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Hydroxybenzoates
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Ligands
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Receptors, Cytoplasmic and Nuclear
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Receptors, Retinoic Acid
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Retinoid X Receptors
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Transcription Factors
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Xenopus Proteins
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benzoate X receptor, Xenopus
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Dexamethasone
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DNA
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Methylprednisolone