Purification and in vitro activity of Rho-associated kinase

Methods Enzymol. 2000:325:149-55. doi: 10.1016/s0076-6879(00)25439-6.

Authors

M Amano¹, Y Fukata, H Shimokawa, K Kaibuchi

Affiliation

¹ Division of Signal Transduction, Nara Institute of Science and Technology, Japan.

PMID: 11036600
DOI: 10.1016/s0076-6879(00)25439-6

No abstract available

MeSH terms

1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / analogs & derivatives
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology
Amides / pharmacology
Animals
Baculoviridae / genetics
Catalytic Domain / genetics
Cattle
Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
Intracellular Signaling Peptides and Proteins
Protein Serine-Threonine Kinases / antagonists & inhibitors
Protein Serine-Threonine Kinases / isolation & purification
Protein Serine-Threonine Kinases / metabolism*
Pyridines / pharmacology
Recombinant Fusion Proteins / metabolism
Staurosporine / pharmacology
rho-Associated Kinases
rhoA GTP-Binding Protein / metabolism*

Substances

Amides
Intracellular Signaling Peptides and Proteins
Pyridines
Recombinant Fusion Proteins
Y 27632
Guanosine 5'-O-(3-Thiotriphosphate)
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Protein Serine-Threonine Kinases
rho-Associated Kinases
rhoA GTP-Binding Protein
Staurosporine
fasudil