Abstract
Orexins are two recently discovered neuropeptides that play an important role in the regulation of food intake and in the regulation of the sleep-wake cycle. In this work we examined the effects of thyroid hormones on prepro-OX expression in the rat hypothalamus, and OXRs expression in the rat hypothalamus and adrenal gland. Hypo- and hyperthyroidism were induced in adult male rats, and the levels of hypothalamic prepro-OX and OXRs mRNA, and adrenal OXRs mRNA were determined using semiquantitative reverse transcription-polymerase chain reaction and/or in situ hybridization. Our results indicate that thyroid status affects neither prepro-OX in the hypothalamus nor hypothalamic and adrenal gland OXRs expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenal Glands / metabolism*
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Amitrole / pharmacology
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Animals
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Enzyme Inhibitors / pharmacology
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Hyperthyroidism / chemically induced
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Hyperthyroidism / metabolism*
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Hypothalamus / metabolism*
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Hypothyroidism / chemically induced
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Hypothyroidism / metabolism*
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Intracellular Signaling Peptides and Proteins
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Male
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Neuropeptides*
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Orexin Receptors
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Orexins
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Protein Precursors / metabolism*
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Receptors, G-Protein-Coupled
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Receptors, Neuropeptide / metabolism*
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Thyroid Gland / drug effects
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Thyroid Gland / metabolism
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Thyrotropin / blood
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Thyrotropin / drug effects
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Thyroxine / pharmacology
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Weight Gain / drug effects
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Weight Gain / physiology
Substances
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Enzyme Inhibitors
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Intracellular Signaling Peptides and Proteins
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Neuropeptides
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Orexin Receptors
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Orexins
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Protein Precursors
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RNA, Messenger
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Receptors, G-Protein-Coupled
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Receptors, Neuropeptide
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Thyrotropin
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Thyroxine
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Amitrole