Abstract
The effects of tunicamycin, a specific inhibitor of N-linked glycosylation, on functional expression of glutamate receptor subtypes were investigated in RNA-injected oocytes. In the presence of tunicamycin the expression of ligand-operated receptors sensitive to kainate, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) and quisqualate were completely blocked. The inhibitory effect was reversible after removal of tunicamycin from the culture medium.
MeSH terms
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Animals
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Electrophysiology
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Excitatory Amino Acid Antagonists*
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Ibotenic Acid / analogs & derivatives
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Ibotenic Acid / pharmacology
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Ion Channels / drug effects
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Ion Channels / metabolism
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Kainic Acid / pharmacology
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Oligosaccharides / metabolism
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Oocytes / drug effects
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Oocytes / metabolism*
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Quisqualic Acid / pharmacology
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RNA / biosynthesis
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Receptors, Glutamate / biosynthesis
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Tunicamycin / pharmacology*
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Xenopus laevis
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Substances
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Excitatory Amino Acid Antagonists
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Ion Channels
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Oligosaccharides
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Receptors, Glutamate
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Tunicamycin
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Ibotenic Acid
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RNA
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
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Quisqualic Acid
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Kainic Acid