Abstract
The effects of inhibitors of nitric oxide (NO) synthase and other cationic amino acids on unidirectional L-arginine transport were studied in porcine aortic endothelial cells cultured in microwell plates or perfused in microcarrier columns. L-Homoarginine, L-lysine and L-ornithine inhibited transport of L-arginine. The NO synthase inhibitors NG-monomethyl-L-arginine and NG-iminoethyl-L-ornithine also reduced L-arginine uptake, whereas NG-nitro-L-arginine and its methyl-ester had no inhibitory effect. The ability to modulate selectively endothelial cell L-arginine transport or NO synthase activity will allow further characterization of the arginine transporter and its role in regulating NO biosynthesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Oxidoreductases / antagonists & inhibitors*
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Amino Acid Transport Systems
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Animals
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Arginine / analogs & derivatives
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Arginine / metabolism*
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Arginine / pharmacology
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Biological Transport, Active / drug effects
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Carrier Proteins / metabolism
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Cells, Cultured
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / metabolism*
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NG-Nitroarginine Methyl Ester
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Nitric Oxide Synthase
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Nitroarginine
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Ornithine / analogs & derivatives
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Ornithine / pharmacology
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omega-N-Methylarginine
Substances
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Amino Acid Transport Systems
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Carrier Proteins
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Nitroarginine
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omega-N-Methylarginine
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N(G)-iminoethylornithine
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Arginine
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Ornithine
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Nitric Oxide Synthase
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Amino Acid Oxidoreductases
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NG-Nitroarginine Methyl Ester