Abstract
Eicosanoid formation by transcellular routes can amplify the levels and types of lipid mediators within a local milieu. To evaluate the role of adhesion in this process, we assessed the influence of mAb against adhesion molecules on LTC4 generation by PMN-endothelial cell interaction. Transcellular LTC4 generation was initiated by addition of fMLP to coincubations of GM-CSF-primed PMN and TNF-activated endothelial cells cultured from kidney glomeruli. Both PMN-endothelial cell adhesion and transcellular LTC4 generation were inhibited by mAb against leukocyte L-selectin and CD18. These results indicate that cytokine-treated PMN and endothelial cells generate LTC4 via transcellular routes by receptor-triggered mechanisms. They suggest that adhesion promotes transcellular eicosanoid biosynthesis and that adhesion molecules may also be targets for blockade of transcellular biosynthesis of lipid mediators.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal*
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Antigens, CD / immunology
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Antigens, CD / physiology*
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CD18 Antigens
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Cell Adhesion Molecules / immunology
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Cell Adhesion Molecules / physiology*
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Cell Adhesion*
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Cell Communication
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Cells, Cultured
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / physiology*
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
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Humans
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In Vitro Techniques
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Kidney Glomerulus / blood supply
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Kinetics
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L-Selectin
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Neutrophils / drug effects
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Neutrophils / physiology*
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Receptors, Leukocyte-Adhesion / drug effects
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Receptors, Leukocyte-Adhesion / physiology
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Recombinant Proteins / pharmacology
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SRS-A / biosynthesis*
Substances
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Antibodies, Monoclonal
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Antigens, CD
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CD18 Antigens
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Cell Adhesion Molecules
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Receptors, Leukocyte-Adhesion
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Recombinant Proteins
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SRS-A
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L-Selectin
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N-Formylmethionine Leucyl-Phenylalanine
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Granulocyte-Macrophage Colony-Stimulating Factor