Abstract
The synthesis of a new class of antisense oligonucleotide compounds with 3'-3' and 5'-5' end inversion (INV-oligonucleotides) is described. Besides the advantage of simplicity of synthesis, physico-chemical studies show that these compounds do not disturb Watson-Crick base-pairing. INV-oligonucleotides have a half-life of 30 h in human serum. We show that they are capable of inhibiting SV40 large T-antigen expression in COS-1 cells, both in vitro and in vivo, and by modulation of the expression of cellular oncoprotein p53 in vitro.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Polyomavirus Transforming / genetics
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Base Sequence
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Cell Line
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Gene Expression / drug effects*
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Half-Life
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Humans
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Oligonucleotides, Antisense / chemical synthesis*
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Oligonucleotides, Antisense / metabolism
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Oligonucleotides, Antisense / pharmacology
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Precipitin Tests
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Protein Biosynthesis / drug effects
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Tumor Suppressor Protein p53 / genetics
Substances
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Antigens, Polyomavirus Transforming
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Oligonucleotides, Antisense
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Tumor Suppressor Protein p53