Abstract
The mechanism of transport of salicylic acid and five other hydroxybenzoic acids across the human erythrocyte membrane was investigated. The specific anion transport inhibitor, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, reduced but did not totally abolish transport of these acids. This observation suggests that these acids are transported by two parallel processes, one involving the band 3 anion transport protein channel and the other probably involving passive diffusion of the un-ionized molecule. The activation energies for membrane transport were large (99-127 kJ.mol-1), an observation that is consistent with the rate-limiting step for anion transport being the conformational change of the band 3 anion transport protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
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4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / analogs & derivatives
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4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / pharmacology
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Anion Exchange Protein 1, Erythrocyte / antagonists & inhibitors
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Anion Exchange Protein 1, Erythrocyte / physiology*
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Anion Transport Proteins
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Biological Transport
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Carrier Proteins / antagonists & inhibitors
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Carrier Proteins / physiology*
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Erythrocyte Membrane / metabolism*
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Humans
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Hydroxybenzoates / pharmacokinetics*
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Kinetics
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Salicylates / pharmacokinetics*
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Salicylic Acid
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Structure-Activity Relationship
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Temperature
Substances
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Anion Exchange Protein 1, Erythrocyte
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Anion Transport Proteins
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Carrier Proteins
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Hydroxybenzoates
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Salicylates
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4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
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Salicylic Acid
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid