Abstract
In a rat kindling model, we examined expression patterns of NKCC1, KCC1, KCC2, and CLC-2. In the dentate granule cell layer, there was an activity-dependent increase in NKCC1 mRNA but significant decreases in KCC1 and CLC-2 mRNAs. In addition, CLC-2 mRNA expression was markedly decreased in CA1 pyramidal layer. These results suggest that an increase in [Cl-]i and a resultant reduction in GABAergic inhibition may occur in hippocampus of epileptic rats.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amygdala / physiology*
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Animals
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Autoradiography
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CLC-2 Chloride Channels
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Chloride Channels / biosynthesis
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Chloride Channels / genetics
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Chlorides / metabolism*
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Gene Expression Regulation / physiology*
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Hippocampus / metabolism*
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Homeostasis / genetics*
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K Cl- Cotransporters
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Kindling, Neurologic / physiology*
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Male
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Pyramidal Cells / metabolism
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Rats
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Rats, Wistar
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Sodium-Potassium-Chloride Symporters / biosynthesis
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Sodium-Potassium-Chloride Symporters / genetics
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Solute Carrier Family 12, Member 2
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Symporters / biosynthesis
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Symporters / genetics
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gamma-Aminobutyric Acid / physiology
Substances
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CLC-2 Chloride Channels
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Chloride Channels
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Chlorides
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RNA, Messenger
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Slc12a2 protein, rat
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Sodium-Potassium-Chloride Symporters
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Solute Carrier Family 12, Member 2
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Symporters
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gamma-Aminobutyric Acid