Stimulation of renal Na+ dicarboxylate cotransporter 1 by Na+/H+ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B

Christoph Boehmer; Hamdy M Embark; Anna Bauer; Monica Palmada; Chris H Yun; Edward J Weinman; Hitoshi Endou; Philip Cohen; Sven Lahme; Karl-Horst Bichler; Florian Lang

doi:10.1016/j.bbrc.2003.12.011

Stimulation of renal Na+ dicarboxylate cotransporter 1 by Na+/H+ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B

Biochem Biophys Res Commun. 2004 Jan 23;313(4):998-1003. doi: 10.1016/j.bbrc.2003.12.011.

Authors

Christoph Boehmer¹, Hamdy M Embark, Anna Bauer, Monica Palmada, Chris H Yun, Edward J Weinman, Hitoshi Endou, Philip Cohen, Sven Lahme, Karl-Horst Bichler, Florian Lang

Affiliation

¹ Department of Physiology I, University of Tübingen, Tübingen D-72076, Germany.

PMID: 14706641
DOI: 10.1016/j.bbrc.2003.12.011

Abstract

Renal tubular citrate transport is accomplished by electrogenic Na(+) coupled dicarboxylate transporter NaDC-1, a carrier subjected to regulation by acidosis. Trafficking of the Na(+)/H(+) exchanger NHE3 is controlled by NHE regulating factors NHERF-1 and NHERF-2 and the serum and glucocorticoid inducible kinase SGK1. To test for a possible involvement in NaDC-1 regulation, mRNA encoding NaDC-1 was injected into Xenopus oocytes with or without cRNA encoding NHERF-1, NHERF-2, SGK1, SGK2, SGK3, and/or the constitutively active form of the related protein kinase B ((T308,S473D)PKB). Succinate induced inward currents (I(succ)) were taken as a measure of transport rate. Coexpression of neither NHERF-1 nor NHERF-2 in NaDC-1 expressing oocytes significantly altered I(succ). On the other hand, coexpression of SGK1, SGK3, and (T308,S473D)PKB stimulated I(succ), an effect further stimulated by additional coexpression of NHERF-2 but not of NHERF-1. The action of the kinases and NHERF-2 may link urinary citrate excretion to proximal tubular H(+) secretion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Citric Acid / metabolism
Cytoskeletal Proteins / genetics
Cytoskeletal Proteins / metabolism*
Dicarboxylic Acid Transporters / genetics
Dicarboxylic Acid Transporters / metabolism*
Female
Humans
Immediate-Early Proteins
In Vitro Techniques
Kidney / metabolism*
Kidney Tubules, Proximal / metabolism
Kinetics
Nuclear Proteins*
Oocytes / drug effects
Oocytes / metabolism
Organic Anion Transporters, Sodium-Dependent / genetics
Organic Anion Transporters, Sodium-Dependent / metabolism*
Phosphoproteins / genetics
Phosphoproteins / metabolism
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism*
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins c-akt
Rats
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sodium-Hydrogen Exchangers
Succinic Acid / pharmacology
Symporters / genetics
Symporters / metabolism*
Xenopus laevis

Substances

Cytoskeletal Proteins
Dicarboxylic Acid Transporters
Immediate-Early Proteins
Nuclear Proteins
Organic Anion Transporters, Sodium-Dependent
Phosphoproteins
Proto-Oncogene Proteins
Recombinant Proteins
SLC13A2 protein, human
Slc13a2 protein, rat
Slc9a3r2 protein, rat
Sodium-Hydrogen Exchangers
Symporters
sodium-hydrogen exchanger regulatory factor
Citric Acid
Succinic Acid
Protein Serine-Threonine Kinases
Proto-Oncogene Proteins c-akt
serum-glucocorticoid regulated kinase