Peripheral amylin activates circumventricular organs expressing calcitonin receptor a/b subtypes and receptor-activity modifying proteins in the rat

Stephan W Barth; Thomas Riediger; Thomas A Lutz; Gerhard Rechkemmer

doi:10.1016/j.brainres.2003.10.040

Peripheral amylin activates circumventricular organs expressing calcitonin receptor a/b subtypes and receptor-activity modifying proteins in the rat

Brain Res. 2004 Jan 30;997(1):97-102. doi: 10.1016/j.brainres.2003.10.040.

Authors

Stephan W Barth¹, Thomas Riediger, Thomas A Lutz, Gerhard Rechkemmer

Affiliation

¹ Federal Research Centre for Nutrition, Institute of Nutritional Physiology, Haid und Neu Str. 9, 76131 Karlsruhe, Germany. stephan.barth@bfe.uni-karlsruhe.de

PMID: 14715154
DOI: 10.1016/j.brainres.2003.10.040

Abstract

The pancreatic hormone amylin (AMY) and the AMY-receptor-agonist salmon-calcitonin (sCT) reduce short-term food-intake after binding to the area postrema (AP), a circumventricular organ (CVO) lacking blood-brain-barrier characteristics. AMY has also been proposed to induce drinking via another CVO, the subfornical organ (SFO). In cellular systems, AMY-binding is generated by interaction of calcitonin-receptor a/b (CT((a))/CT((b))) with receptor-activity modifying proteins (RAMPs). By using in situ hybridization, the codistribution of CT((a))/CT((b)) with RAMP1-3 and c-fos was mapped in CVOs of rats. AMY and sCT induced c-fos within the SFO which contained CT((a)) and/or CT((b)) and RAMP1/2 mRNA. AMY and sCT also activated AP neurons, which express the CT((a)), but not the CT((b)), receptor and RAMP2/3 mRNA. These data emphasize the important role of these structures as primary targets for circulating AMY.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amyloid / metabolism*
Animals
Anti-Asthmatic Agents / pharmacology
Behavior, Animal
Calcitonin / pharmacology
Eating / drug effects
Gene Expression / drug effects
In Situ Hybridization / methods
Intracellular Signaling Peptides and Proteins
Islet Amyloid Polypeptide
Male
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Proto-Oncogene Proteins c-fos / genetics
Proto-Oncogene Proteins c-fos / metabolism
Rats
Rats, Wistar
Receptor Activity-Modifying Protein 1
Receptor Activity-Modifying Protein 2
Receptor Activity-Modifying Proteins
Receptors, Calcitonin / genetics
Receptors, Calcitonin / metabolism*
Subfornical Organ / metabolism*

Substances

Amyloid
Anti-Asthmatic Agents
Intracellular Signaling Peptides and Proteins
Islet Amyloid Polypeptide
Membrane Proteins
Proto-Oncogene Proteins c-fos
Ramp1 protein, rat
Ramp2 protein, rat
Receptor Activity-Modifying Protein 1
Receptor Activity-Modifying Protein 2
Receptor Activity-Modifying Proteins
Receptors, Calcitonin
salmon calcitonin
Calcitonin