Abstract
1. The present study was aimed at elucidating effects of transforming growth factor-beta (TGF-beta) on blood-brain barrier (BBB) functions with mouse brain capillary endothelial (MBEC4) cells. 2. The permeability coefficients of sodium fluorescein and Evans blue albumin for MBEC4 cells and the cellular accumulation of rhodamine 123 in MBEC4 cells were dose-dependently decreased after a 12-h exposure to TGF-beta1 (0.01-10 ng/mL). 3. The present study demonstrates that TGF-beta lowers the endothelial permeability and enhances the functional activity of P-gp, suggesting that cellular constituents producing TGF-beta in the brain may keep the BBB functioning.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
ATP Binding Cassette Transporter, Subfamily B, Member 1 / drug effects
-
ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
-
Animals
-
Blood-Brain Barrier / drug effects
-
Blood-Brain Barrier / physiology*
-
Cell Line
-
Cell Membrane / drug effects
-
Cell Membrane / metabolism
-
Cell Membrane Permeability / drug effects
-
Cell Membrane Permeability / physiology
-
Endothelial Cells / cytology
-
Endothelial Cells / drug effects
-
Endothelial Cells / metabolism*
-
Evans Blue / pharmacokinetics
-
Fluorescein / pharmacokinetics
-
Mice
-
Mice, Inbred BALB C
-
Microcirculation / cytology
-
Microcirculation / drug effects
-
Microcirculation / metabolism*
-
Rhodamines / pharmacokinetics
-
Tight Junctions / drug effects
-
Tight Junctions / metabolism*
-
Transforming Growth Factor beta / metabolism*
-
Transforming Growth Factor beta / pharmacology
-
Transforming Growth Factor beta1
-
Up-Regulation / drug effects
-
Up-Regulation / physiology
Substances
-
ATP Binding Cassette Transporter, Subfamily B, Member 1
-
Rhodamines
-
Tgfb1 protein, mouse
-
Transforming Growth Factor beta
-
Transforming Growth Factor beta1
-
Evans Blue
-
Fluorescein