Abstract
Based on the published nucleotide sequence for rat hepatocyte nuclear factor 4 (HNF-4; Sladek, F.M., Zhong, W., Lai, E. and Darnell, J.E., Jr. (1990) Genes Dev. 4, 2353-2365), we have cloned a cDNA by means of polymerase chain reaction amplification of reverse-transcribed RNA (RT-PCR). Our clone contained an extra segment of 30 bp, which was not found in the previously reported clone, in the coding region near the C-terminus. Further RT-PCR analysis demonstrated that both isoforms of HNF-4 mRNA, i.e., with or without the 30 nucleotide segment, occur in rat liver and kidney, presumably by differential splicing.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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DNA
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DNA-Binding Proteins*
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Electrophoresis, Polyacrylamide Gel
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Hepatocyte Nuclear Factor 4
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Liver / metabolism*
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Male
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Molecular Sequence Data
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Phosphoproteins*
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Polymerase Chain Reaction
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Rats
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Rats, Inbred Strains
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Transcription Factors / genetics*
Substances
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DNA-Binding Proteins
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Hepatocyte Nuclear Factor 4
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Phosphoproteins
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Transcription Factors
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DNA
Associated data
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GENBANK/D10554
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GENBANK/M80474
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GENBANK/M80475
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GENBANK/M80476
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GENBANK/M80530
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GENBANK/M80531
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GENBANK/M80532
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GENBANK/M80533
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GENBANK/M91429
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GENBANK/M91430