Abstract
Allosteric modulation of mouse 5-Hydroxytryptamine(3A) (5-HT(3A)) and 5-HT(3A/B) receptor function by ethanol and trichloroethanol (TCEt) was assessed in HEK293 cells with whole cell patch-clamp electrophysiological recordings. Ethanol enhanced 5-HT(3A) receptor function, but had no effect on mouse 5-HT(3A/B) receptor mediated currents. The enhancing action of trichloroethanol (TCEt) on mouse 5-HT(3A/B) receptor function was much less than that observed in the mouse 5-HT(3A) receptor. Where alcohol-induced increases in peak amplitude were observed, the slope of the 20-80% rising phase of current onset was also enhanced, suggesting that increases in activation rate may be one mechanism through which alcohols enhance function of the 5-HT(3) receptors.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Cell Line
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Central Nervous System Depressants / pharmacology*
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Dose-Response Relationship, Drug
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Electric Stimulation / methods
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Ethanol / pharmacology*
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Ethylene Chlorohydrin / analogs & derivatives
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Ethylene Chlorohydrin / pharmacology
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Gene Expression / drug effects*
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Humans
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Membrane Potentials / radiation effects
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Mice
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Patch-Clamp Techniques / methods
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Protein Subunits / genetics
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Protein Subunits / metabolism
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Receptors, Serotonin, 5-HT3 / genetics
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Receptors, Serotonin, 5-HT3 / metabolism*
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Serotonin / pharmacology
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Transfection / methods
Substances
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Central Nervous System Depressants
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Protein Subunits
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Receptors, Serotonin, 5-HT3
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Serotonin
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Ethanol
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Ethylene Chlorohydrin
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2,2,2-trichloroethanol