Abstract
Vasoactive intestinal peptide (VIP) is a neuropeptide which also interacts with cells of the immune system. The paucity of specific VIP receptor antagonists has hampered studies of possible receptor heterogeneity and of VIP function. To aid in achieving these goals, a new VIP antagonist, a hybrid between neurotensin and VIP, has been synthesized. This peptide interacted with VIP receptors on spinal cord cells with an affinity 10-fold greater than VIP itself. In contrast, 1000-fold higher concentrations of the antagonist were required to displace labeled VIP from its receptor on lymphoid cells as compared to VIP itself, suggesting VIP receptor heterogeneity between immune and spinal cord cells.
MeSH terms
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Amino Acid Sequence
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Animals
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Binding, Competitive
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Cells, Cultured
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DNA Replication / drug effects
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Kinetics
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Lymph Nodes / immunology
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Lymph Nodes / metabolism
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Lymphocyte Activation / drug effects
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Lymphocytes / drug effects
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Lymphocytes / immunology
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Lymphocytes / metabolism*
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Molecular Sequence Data
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Neurotensin
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Receptors, Gastrointestinal Hormone / drug effects
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Receptors, Gastrointestinal Hormone / metabolism*
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Receptors, Vasoactive Intestinal Peptide
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Spinal Cord / metabolism*
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Vasoactive Intestinal Peptide / antagonists & inhibitors*
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Vasoactive Intestinal Peptide / metabolism
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Vasoactive Intestinal Peptide / pharmacology
Substances
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Receptors, Gastrointestinal Hormone
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Receptors, Vasoactive Intestinal Peptide
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Vasoactive Intestinal Peptide
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Neurotensin