Abstract
A novel generation of silk-based brain implants engineered to release adenosine was recently shown to provide robust seizure suppression in kindled rats. As a first step to develop stem cell-coated silk-based 3D-scaffolds for the therapeutic long-term delivery of adenosine we engineered human mesenchymal stem cells (hMSCs) to release adenosine. Here we demonstrate reduction of chronic seizures in a mouse model of CA3-selective epileptogenesis after infrahippocampal transplantation of adenosine-releasing hMSCs.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adenosine / biosynthesis
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Adenosine / therapeutic use*
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Adenosine Kinase / antagonists & inhibitors
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Adenosine Kinase / genetics
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Analysis of Variance
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Animals
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Disease Models, Animal
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Electroencephalography / methods
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Green Fluorescent Proteins / genetics
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Hippocampus / surgery
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Humans
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Indoles
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Kainic Acid
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Mesenchymal Stem Cell Transplantation / methods*
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Mesenchymal Stem Cells / physiology*
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Mice
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MicroRNAs / genetics
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Seizures / chemically induced
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Seizures / drug therapy*
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Tissue Engineering / methods*
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Transfection / methods
Substances
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Indoles
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MicroRNAs
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Green Fluorescent Proteins
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DAPI
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Adenosine Kinase
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Adenosine
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Kainic Acid