Abstract
A mutant pyrrolysyl-tRNA synthetase/tRNA pair was used to genetically encode allylcarbamoyl methyllysine in bacteria. This amino acid can be converted to methyllysine with a ruthenium catalyst, providing a straightforward approach for site-specifically introducing methyllysine residues into proteins.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acyl-tRNA Synthetases / genetics*
-
Escherichia coli / genetics*
-
Escherichia coli Proteins / genetics*
-
Lysine / analogs & derivatives*
-
Lysine / genetics
-
Mutation
-
RNA, Transfer / genetics*
Substances
-
Escherichia coli Proteins
-
2-methyllysine
-
RNA, Transfer
-
Amino Acyl-tRNA Synthetases
-
Lysine