Abstract
We investigated the expression of the partially duplicated α7 nAChR subunit gene in three monocytic cell lines: THP-1, U937 and Mono-Mac-6. Qualitative PCR revealed the presence of the classic α7 gene in these lineages, but real-time PCR showed the exclusive expression of α7dup. Both mRNA and protein levels were reduced in THP-1 upon LPS challenge, and it was found that transcriptional down-regulation was mediated by a direct mechanism dependent on NF-κB as its specific inhibitor parthenolide prevented the reduction in the α7dup transcript. Such precise regulation suggests that α7dup may specifically participate in the inflammatory response of the innate immune system.
Copyright © 2010 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Down-Regulation
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Enzyme-Linked Immunosorbent Assay
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Gene Expression Regulation / immunology*
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Genes, Duplicate
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Humans
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Immunoblotting
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Inflammation / chemically induced
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Inflammation / genetics*
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Lipopolysaccharides / adverse effects
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Lipopolysaccharides / immunology
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Monocytes / immunology*
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Monocytes / metabolism
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Protein Isoforms / biosynthesis
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Protein Isoforms / genetics
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Receptors, Nicotinic / biosynthesis*
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Receptors, Nicotinic / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Transcription, Genetic
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alpha7 Nicotinic Acetylcholine Receptor
Substances
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Chrna7 protein, human
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Lipopolysaccharides
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Protein Isoforms
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Receptors, Nicotinic
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alpha7 Nicotinic Acetylcholine Receptor