Abstract
Based on the concept that anticocaine antibodies could prevent inhaled cocaine from reaching its target receptors in the brain, an effective anticocaine vaccine could help reverse cocaine addiction. Leveraging the knowledge that E1(-)E3(-) adenovirus (Ad) gene transfer vectors are potent immunogens, we have developed a novel vaccine platform for addictive drugs by covalently linking a cocaine analog to the capsid proteins of noninfectious, disrupted Ad vector. The Ad-based anticocaine vaccine evokes high-titer anticocaine antibodies in mice sufficient to completely reverse, on a persistent basis, the hyperlocomotor activity induced by intravenous administration of cocaine.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adenoviridae / genetics
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Animals
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Antibodies / blood
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Cocaine / analogs & derivatives*
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Cocaine / immunology*
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Cocaine / metabolism
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Defective Viruses / genetics
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Defective Viruses / immunology
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Defective Viruses / metabolism
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Female
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Gene Transfer Techniques
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Genetic Vectors / administration & dosage
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Genetic Vectors / genetics
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Locomotion / drug effects
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Locomotion / immunology
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Mice
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Mice, Inbred BALB C
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Motor Activity / drug effects
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Motor Activity / immunology
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Substance-Related Disorders* / immunology
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Substance-Related Disorders* / prevention & control
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Vaccines* / administration & dosage
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Vaccines* / immunology
Substances
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Antibodies
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Vaccines
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Cocaine