Abstract
Lipoxins (LX) are trihydroxytetraene metabolites derived from arachidonic acid via an interaction between the 5- and 15-lipoxygenases. Preincubation of [3H] myo-inositol labeled PMN with 10-7M and 10-5M LXA4 for 1 minute at 37 degrees C resulted in a concentration dependent inhibition of the generation of [3H] IP3 and [3H] IP in cells subsequently stimulated by increasing doses of LTB4 or FMLP for 1 minute at 37 degrees C. Preincubation of PMN with LXA4 did not inhibit specific binding of [3H] LTB4 to PMN. These results indicate that LXA4 inhibits chemotactic factor-induced phosphoinositide hydrolysis at a post-receptor level.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Dose-Response Relationship, Drug
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Humans
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Hydrolysis
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Hydroxyeicosatetraenoic Acids / pharmacology*
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In Vitro Techniques
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Inositol / blood
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Inositol 1,4,5-Trisphosphate / blood
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Inositol Phosphates / blood
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Kinetics
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Leukotriene B4 / blood
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Leukotriene B4 / pharmacology
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Lipoxins*
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Neutrophils / drug effects
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Neutrophils / metabolism*
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Phosphatidylinositols / blood*
Substances
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Hydroxyeicosatetraenoic Acids
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Inositol Phosphates
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Lipoxins
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Phosphatidylinositols
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lipoxin A4
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Leukotriene B4
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Inositol
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N-Formylmethionine Leucyl-Phenylalanine
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Inositol 1,4,5-Trisphosphate