Abstract
The Toll gene of Drosophila, a maternal effect gene that plays a central role in the establishment of the embryonic dorsal-ventral pattern, has been cloned using P element tagging. A 5.3 kb poly(A)+ ovarian transcript from the cloned region was purified by hybrid selection with the cloned DNA. This purified transcript complements the Toll mutant phenotype when injected into Toll- embryos, which proves that it is the Toll transcript. The sequence of cDNAs suggests that the Toll protein is an integral membrane protein with a cytoplasmic domain and a large extracytoplasmic domain. The putative extracytoplasmic domain contains at least 15 repeats of a 24 amino acid, leucine-rich sequence found in both human and yeast membrane proteins.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Amino Acid Sequence
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Animals
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Base Sequence
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Chromosomes / ultrastructure
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DNA / genetics
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DNA / isolation & purification
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DNA Transposable Elements
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DNA, Recombinant / isolation & purification
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Drosophila / embryology
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Drosophila / genetics*
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Female
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Hybridization, Genetic
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Membrane Proteins / genetics*
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Molecular Sequence Data
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Mutation
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Nucleic Acid Hybridization
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Phenotype
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Poly A / genetics
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RNA / genetics
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RNA, Messenger
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Repetitive Sequences, Nucleic Acid
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Transcription, Genetic
Substances
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DNA Transposable Elements
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DNA, Recombinant
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Membrane Proteins
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RNA, Messenger
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Poly A
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RNA
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DNA
Associated data
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GENBANK/J02682
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GENBANK/M19969