A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II

M Noda; H Suzuki; S Numa; W Stühmer

doi:10.1016/0014-5793(89)81531-5

A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II

FEBS Lett. 1989 Dec 18;259(1):213-6. doi: 10.1016/0014-5793(89)81531-5.

Authors

M Noda¹, H Suzuki, S Numa, W Stühmer

Affiliation

¹ Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.

PMID: 2557243
DOI: 10.1016/0014-5793(89)81531-5

Abstract

A single point mutation of the rat sodium channel II reduces its sensitivity to tetrodotoxin and saxitoxin by more than three orders of magnitude. The mutation replaces glutamic acid 387 with a glutamine and has only slight effects on the macroscopic current properties, as measured under voltage-clamp in Xenopus oocytes injected with the corresponding cDNA-derived mRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Electric Conductivity
Membrane Potentials
Mutation
Rats
Saxitoxin / pharmacology*
Sodium Channels / drug effects*
Sodium Channels / physiology
Sodium Channels / ultrastructure
Structure-Activity Relationship
Tetrodotoxin / pharmacology*
Xenopus laevis

Substances

Sodium Channels
Saxitoxin
Tetrodotoxin