Abstract
5-Hydroxyeicosatetraenoate (5-HETE) stimulated human neutrophils to elevate their cytosolic Ca2+. It also enhanced the actions of phorbol myristate acetate and a diacylglycerol in stimulating these cells to: a) translocate protein kinase C from cytosol to membranes; b) generate superoxide anion; and c) release granule-bound enzymes. 5,20-DiHETE and 15-HETE possessed little or none of these bioactions. 5-HETE may influence neutrophil function by elevating cytosolic Ca2+ and thereby promoting the mobilization of protein kinase C.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Caenorhabditis elegans Proteins*
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Calcium / blood*
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Carrier Proteins
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Cytosol / metabolism
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Humans
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Hydroxyeicosatetraenoic Acids / pharmacology*
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Kinetics
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Neutrophils / drug effects
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Neutrophils / immunology
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Neutrophils / metabolism*
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Oxygen / blood
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Protein Kinase C / blood*
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Protein Kinase C / metabolism
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Receptors, Drug*
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Tetradecanoylphorbol Acetate / metabolism
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Tetradecanoylphorbol Acetate / pharmacology
Substances
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Caenorhabditis elegans Proteins
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Carrier Proteins
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Hydroxyeicosatetraenoic Acids
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Receptors, Drug
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phorbol ester binding protein
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phorbol ester receptor
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5-hydroxy-6,8,11,14-eicosatetraenoic acid
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Protein Kinase C
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Tetradecanoylphorbol Acetate
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Oxygen
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Calcium