Abstract
We demonstrate in this paper that bupivacaine, a local anesthetic, can act alone as an uncoupler of rat liver mitochondria. It stimulates state 4 respiration, induces a swelling in potassium acetate (in the presence of valinomycin), and collapses the transmembrane potential. Lidocaine, another local anesthetic, requires the presence of a lipophilic anion such as TPB- to produce the same effects. TPB- can also reinforce the action of bupivacaine. These differences in action of the two local anesthetics can be explained by the difference in the liposolubility.
MeSH terms
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Animals
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Bupivacaine / pharmacology*
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Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
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Intracellular Membranes / drug effects
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Intracellular Membranes / physiology
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Kinetics
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Lidocaine / pharmacology*
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Membrane Potentials / drug effects
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Mitochondria, Liver / drug effects
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Mitochondria, Liver / metabolism*
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Mitochondrial Swelling / drug effects
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Oxygen Consumption / drug effects*
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Rats
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Rats, Inbred Strains
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Uncoupling Agents*
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Valinomycin / pharmacology
Substances
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Uncoupling Agents
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Valinomycin
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Carbonyl Cyanide m-Chlorophenyl Hydrazone
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Lidocaine
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Bupivacaine