Abstract
Active human cyclooxygenase-2 (Cox-2) was expressed at high levels in insect cells using a recombinant baculovirus. The specific activity of Cox-2 in the microsomes of infected cells was 0.51 mumol O2/min/mg and was comparable to that obtained for partially purified Cox-2 from ovine placenta (0.55 mumol O2/min/mg). The Cox-2 enzyme expressed in insect cells was glycosylated to varying extents and most of the cyclooxygenase activity was in the high-speed microsomal pellet. The insect-cell-expressed enzyme also showed characteristic 15-hydroxyeicosa-tetraenoic acid production after aspirin treatment and had typical inhibition profiles with a number of known nonsteroidal antiinflammatory drugs.
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology
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Baculoviridae / genetics
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Base Sequence
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Cell Line
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Cloning, Molecular
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Cyclooxygenase Inhibitors / pharmacology
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DNA, Complementary / genetics
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Gene Expression*
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Glycoside Hydrolases / pharmacology
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Glycosylation
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Humans
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Hydroxyeicosatetraenoic Acids / biosynthesis
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Microsomes / enzymology
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Molecular Sequence Data
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Prostaglandin-Endoperoxide Synthases / genetics*
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Prostaglandin-Endoperoxide Synthases / metabolism
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Recombinant Proteins
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Spodoptera / metabolism*
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Transfection
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Cyclooxygenase Inhibitors
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DNA, Complementary
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Hydroxyeicosatetraenoic Acids
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Recombinant Proteins
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15-hydroxy-5,8,11,13-eicosatetraenoic acid
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Prostaglandin-Endoperoxide Synthases
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Glycoside Hydrolases