Abstract
In the present report we analyze by reverse transcription (RT) and polymerase chain reaction (PCR) the expression of vasoactive intestinal peptide receptors (VIP-R) in rat thymocytes and peripheral T and B lymphocytes. We have obtained a specific VIP-R cDNA product of 311 bp identical in size to that obtained from lung. These results have been confirmed by Southern hybridization analysis. VIP receptor gene expression in central and peripheral lymphoid organs support the notion that VIP is a immunomodulatory peptide.
MeSH terms
-
Adjuvants, Immunologic
-
Animals
-
B-Lymphocytes / metabolism
-
Base Sequence
-
Blotting, Southern
-
DNA Primers
-
Gene Expression*
-
Lymph Nodes / metabolism
-
Lymphocytes / metabolism*
-
Molecular Sequence Data
-
Organ Specificity
-
Polymerase Chain Reaction
-
Rats
-
Receptors, Vasoactive Intestinal Peptide / biosynthesis*
-
Spleen / metabolism
-
T-Lymphocytes / metabolism
-
Thymus Gland / metabolism
-
Vasoactive Intestinal Peptide / immunology
Substances
-
Adjuvants, Immunologic
-
DNA Primers
-
Receptors, Vasoactive Intestinal Peptide
-
Vasoactive Intestinal Peptide