Abstract
Analysis of the primary structure of the rabbit skeletal muscle ryanodine receptor led to the identification of two molecules of 5032 and 5037 residues, respectively. Such a sequence discrepancy is likely to be due to the alternative splicing of a 15 bp exon (1) encoding a 5 amino acid insertion (Ala-Gly-Asp-Ala-Gln) after residue 3479. By using PCR on first strand cDNA, we searched for the 15 base pair insertion in the ryanodine receptor mRNA from adult slow- and fast-twitch skeletal muscle, as well as from fast-muscles, at various stages of post-natal development. All rabbit skeletal muscle mRNAs, regardless of their developmental stage and twitch properties, contain two RYR transcripts, suggesting the coexistence of two RYR isoforms in mammalian skeletal muscle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aging / metabolism*
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Alternative Splicing*
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Amino Acid Sequence
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Animals
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Animals, Newborn
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Base Sequence
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Calcium Channels / analysis
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Calcium Channels / biosynthesis*
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DNA Primers
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Exons
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Gene Expression*
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Molecular Sequence Data
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Muscle Development
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Muscle Fibers, Fast-Twitch / growth & development
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Muscle Fibers, Fast-Twitch / metabolism*
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Muscle Fibers, Slow-Twitch / growth & development
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Muscle Fibers, Slow-Twitch / metabolism*
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Muscle Proteins / analysis
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Muscle Proteins / biosynthesis*
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Rabbits
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Ryanodine / metabolism*
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Ryanodine Receptor Calcium Release Channel
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Transcription, Genetic
Substances
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Calcium Channels
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DNA Primers
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Muscle Proteins
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RNA, Messenger
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Ryanodine Receptor Calcium Release Channel
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Ryanodine