Abstract
The nucleotide sequence of a 25.7 kilobase Drosophila melanogaster genomic DNA segment containing a gene for a ryanodine receptor/calcium release channel homologue has been determined. Computer analysis and partial cDNA cloning revealed 26 exons comprising the protein-coding sequence in this gene. The predicted protein is homologous in amino acid sequence and shares characteristic structural features with the mammalian ryanodine receptors. In blot hybridization analysis, a approximately 16 kilobase RNA species was identified abundantly in a 6-12 h embryo as the transcript from this gene. In situ hybridization to polytene chromosomes indicated that this gene locates at band position 44F on the second chromosome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Calcium Channels / chemistry
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Calcium Channels / genetics*
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Chromosome Mapping
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Chromosomes / chemistry
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Cloning, Molecular
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DNA / chemistry
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DNA / isolation & purification*
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Drosophila melanogaster / genetics*
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Endoplasmic Reticulum / chemistry
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In Situ Hybridization
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Molecular Sequence Data
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Muscle Proteins / chemistry
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Muscle Proteins / genetics*
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Nucleic Acid Hybridization
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Repetitive Sequences, Nucleic Acid
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Restriction Mapping
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Ryanodine Receptor Calcium Release Channel
Substances
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Calcium Channels
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DNA, Complementary
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Muscle Proteins
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Ryanodine Receptor Calcium Release Channel
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DNA