Abstract
We have cloned and sequenced a novel cDNA (RPR7) encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP). RPR7 was identified by PCR of rat pituitary cDNA, and full-length clones were isolated from a rat olfactory bulb cDNA library. When expressed in COS cells, RPR7 was functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP) in response to stimulation by PACAP-38, PACAP-27, vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI). The order of potency of these ligands was PACAP-38-PACAP-27 > VIP > PHI, suggesting that the receptor corresponds to the pharmacologically characterised PACAP Type I receptor.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Chlorocebus aethiops
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Cloning, Molecular*
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Cyclic AMP / metabolism
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DNA / chemistry
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DNA / genetics*
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Gene Expression*
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Male
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Molecular Sequence Data
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Neuropeptides / pharmacology
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Olfactory Bulb / chemistry
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Peptide PHI / pharmacology
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Pituitary Adenylate Cyclase-Activating Polypeptide
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Polymerase Chain Reaction
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Rats
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Rats, Wistar
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Receptors, Cell Surface / chemistry
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Receptors, Cell Surface / genetics*
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
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Receptors, Pituitary Hormone*
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Sequence Homology
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Transfection
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Vasoactive Intestinal Peptide / pharmacology
Substances
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Adcyap1 protein, rat
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Adcyap1r1 protein, rat
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Neuropeptides
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Peptide PHI
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Pituitary Adenylate Cyclase-Activating Polypeptide
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Receptors, Cell Surface
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
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Receptors, Pituitary Hormone
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Vasoactive Intestinal Peptide
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DNA
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Cyclic AMP