Abstract
ATP-sensitive K+ (KATP) channels play a crucial role in coupling metabolic energy to the membrane potential of cells. Recently, we have isolated a KATP channel cDNA (uKATP-1) that is expressed ubiquitously in rat tissues including pancreatic islets, pituitary, skeletal muscle, and heart. Here, we report cloning of the human cDNA and gene encoding uKATP-1. Human uKATP-1 is a protein of 424 amino acids exhibiting 98% identity with rat uKATP-1. The human gene encoding uKATP-1, designated KCNJ8, is approximately 9.7 kb in length and is composed of three exons. KCNJ8 was mapped to chromosome 12p11.23 using fluorescence in situ hybridization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP-Binding Cassette Transporters
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Animals
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Base Sequence
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Chromosome Mapping
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Chromosomes, Human, Pair 12*
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Cloning, Molecular
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DNA, Complementary / isolation & purification
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Humans
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In Situ Hybridization, Fluorescence
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KATP Channels
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Molecular Sequence Data
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Potassium Channels / chemistry
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Potassium Channels / genetics*
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Potassium Channels, Inwardly Rectifying
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Rats
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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ATP-Binding Cassette Transporters
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DNA, Complementary
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KATP Channels
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Potassium Channels
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Potassium Channels, Inwardly Rectifying
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uK-ATP-1 potassium channel
Associated data
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GENBANK/D50312
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GENBANK/D50313
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GENBANK/D50314
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GENBANK/D50315