Abstract
A cDNA clone for mouse pituitary adenylate cyclase-activating polypeptide (PACAP) receptor (PACAP-R) was obtained from the brain using reverse transcription-polymerase chain reaction (RT-PCR). The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000-times higher affinity than vasoactive intestinal polypeptide (VIP), and PACAP stimulated adenylate cyclase through the cloned PACAP receptor. The mouse PACAP receptor consists of 496 amino acids, contains seven transmembrane segments and has 98.4%, 93.0%, and 92.5% identity with the rat, bovine, and human PACAP-R, respectively.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cattle
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Cell Line
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Cloning, Molecular*
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DNA, Complementary / chemistry*
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Humans
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Mice
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Molecular Sequence Data
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Neuropeptides / metabolism
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Pituitary Adenylate Cyclase-Activating Polypeptide
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Rats
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
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Receptors, Pituitary Hormone / genetics*
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Receptors, Pituitary Hormone / metabolism
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Recombinant Proteins / metabolism
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Sequence Homology
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Transfection
Substances
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ADCYAP1 protein, human
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Adcyap1 protein, mouse
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Adcyap1 protein, rat
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Adcyap1r1 protein, mouse
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DNA, Complementary
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Neuropeptides
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Pituitary Adenylate Cyclase-Activating Polypeptide
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
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Receptors, Pituitary Hormone
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Recombinant Proteins