Inhibition of human leukocyte proteinase 3 by a novel recombinant serine proteinase inhibitor (LEX032)

W C Groutas; S Ruan; R Kuang; J B Hook; H Sands

doi:10.1006/bbrc.1997.6526

Inhibition of human leukocyte proteinase 3 by a novel recombinant serine proteinase inhibitor (LEX032)

Biochem Biophys Res Commun. 1997 Apr 28;233(3):697-9. doi: 10.1006/bbrc.1997.6526.

Authors

W C Groutas¹, S Ruan, R Kuang, J B Hook, H Sands

Affiliation

¹ Department of Chemistry, Wichita State University, Kansas 67260, USA.

PMID: 9168917
DOI: 10.1006/bbrc.1997.6526

Abstract

The interaction of a bioengineered serpin (LEX032) with human leukocyte proteinase 3 (PR 3) has been investigated. LEX032 was found to be a time-dependent inhibitor of PR 3, forming a highly-stable enzyme-inhibitor complex (Ki 12 nM).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Binding Sites / genetics
Humans
In Vitro Techniques
Kinetics
Leukocytes / enzymology*
Myeloblastin
Recombinant Proteins / genetics
Recombinant Proteins / pharmacology
Serine Endopeptidases / metabolism*
Serine Proteinase Inhibitors / genetics
Serine Proteinase Inhibitors / pharmacology*
Serpins / genetics
Serpins / pharmacology*

Substances

LEX 032
Recombinant Proteins
Serine Proteinase Inhibitors
Serpins
Serine Endopeptidases
Myeloblastin