The transcriptional co-activator p/CIP binds CBP and mediates nuclear-receptor function

Nature. 1997 Jun 12;387(6634):677-84. doi: 10.1038/42652.

Abstract

The functionally conserved proteins CBP and p300 act in conjunction with other factors to activate transcription of DNA. A new factor, p/CIP, has been discovered that is present in the cell as a complex with CBP and is required for transcriptional activity of nuclear receptors and other CBP/p300-dependent transcription factors. The highly related nuclear-receptor co-activator protein NCoA-1 is also specifically required for ligand-dependent activation of genes by nuclear receptors. p/CIP, NCoA-1 and CBP all contain related leucine-rich charged helical interaction motifs that are required for receptor-specific mechanisms of gene activation, and allow the selective inhibition of distinct signal-transduction pathways.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • CREB-Binding Protein
  • Cell Line
  • E1A-Associated p300 Protein
  • Gene Expression Regulation
  • Genes, Reporter
  • HeLa Cells
  • Histone Acetyltransferases
  • Humans
  • Interferon-gamma / antagonists & inhibitors
  • Interferon-gamma / metabolism
  • Leucine / metabolism
  • Molecular Sequence Data
  • Nuclear Proteins / metabolism*
  • Nuclear Receptor Coactivator 1
  • Nuclear Receptor Coactivator 2
  • Nuclear Receptor Coactivator 3
  • Protein Binding
  • Rats
  • Receptors, Cytoplasmic and Nuclear / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcriptional Activation
  • Tretinoin / antagonists & inhibitors
  • Tretinoin / metabolism

Substances

  • Nuclear Proteins
  • Nuclear Receptor Coactivator 2
  • Receptors, Cytoplasmic and Nuclear
  • Recombinant Fusion Proteins
  • Trans-Activators
  • Transcription Factors
  • Tretinoin
  • Interferon-gamma
  • CREB-Binding Protein
  • CREBBP protein, human
  • Crebbp protein, rat
  • E1A-Associated p300 Protein
  • Ep300 protein, rat
  • Histone Acetyltransferases
  • NCOA1 protein, human
  • NCOA3 protein, human
  • Nuclear Receptor Coactivator 1
  • Nuclear Receptor Coactivator 3
  • Leucine

Associated data

  • GENBANK/AF000581
  • GENBANK/AF000582