Abstract
Two cDNAs, size 969 bp and 1146 bp respectively, encoding goldfish gamma-preprotachykinin (gamma-PPT) were identified. Both cDNAs contain the same 345 bp open reading frame. The deduced 114-amino acid gamma-PPT contains the sequence of substance P, carassin and neurokinin A. sequence analysis of the two cDNA 5'-untranslated regions shows that the two cDNAs may represent different PPT-A gene transcripts resulting from the alternative transcriptional start sites. Expression of gamma-PPT mRNA was detected in a wide range of brain areas from the olfactory bulbs to the posterior brain region, as well as in the intestine, testis and pituitary.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Southern
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DNA, Complementary / chemistry
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Fishes
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Goldfish
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Male
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Mammals
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Molecular Sequence Data
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Neurokinin A / genetics*
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Peptide Mapping
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Polymerase Chain Reaction
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Protein Precursors / chemistry
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Protein Precursors / genetics*
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RNA, Messenger / genetics
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Ranidae
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Sequence Alignment
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Substance P / genetics*
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Tachykinins / chemistry
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Tachykinins / genetics*
Substances
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DNA, Complementary
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Protein Precursors
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RNA, Messenger
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Tachykinins
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preprotachykinin
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carassin
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Substance P
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Neurokinin A
Associated data
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GENBANK/U61272
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GENBANK/U63531