Abstract
Amyloid-beta is a neurotoxic peptide which is implicated in the pathogenesis of Alzheimer's disease. It binds an intracellular polypeptide known as ERAB, thought to be a hydroxysteroid dehydrogenase enzyme, which is expressed in normal tissues, but is overexpressed in neurons affected in Alzheimer's disease. ERAB immunoprecipitates with amyloid-beta, and when cell cultures are exposed to amyloid-beta, ERAB inside the cell is rapidly redistributed to the plasma membrane. The toxic effect of amyloid-beta on these cells is prevented by blocking ERAB and is enhanced by overexpression of ERAB. By interacting with intracellular amyloid-beta, ERAB may therefore contribute to the neuronal dysfunction associated with Alzheimer's disease.
MeSH terms
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3-Hydroxyacyl CoA Dehydrogenases*
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Alzheimer Disease / metabolism*
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Alzheimer Disease / pathology
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Amino Acid Sequence
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Amyloid beta-Peptides / metabolism*
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Amyloid beta-Peptides / toxicity
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Animals
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Brain / metabolism
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Brain / pathology
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COS Cells
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cloning, Molecular
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HeLa Cells
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Humans
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Hydroxysteroid Dehydrogenases / genetics
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Hydroxysteroid Dehydrogenases / metabolism
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Molecular Sequence Data
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Protein Binding
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Transfection
Substances
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Amyloid beta-Peptides
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Carrier Proteins
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Recombinant Fusion Proteins
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Hydroxysteroid Dehydrogenases
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3-Hydroxyacyl CoA Dehydrogenases
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HSD17B10 protein, human
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Hsd17b10 protein, mouse