Abstract
The generation of pacemaker activity in heart and brain is mediated by hyperpolarization-activated cation channels that are directly regulated by cyclic nucleotides. We previously cloned a novel member of the voltage-gated K channel family from mouse brain (mBCNG-1) that contained a carboxy-terminal cyclic nucleotide-binding domain (Santoro et al., 1997) and hence proposed it to be a candidate gene for pacemaker channels. Heterologous expression of mBCNG-1 demonstrates that it does indeed code for a channel with properties indistinguishable from pacemaker channels in brain and similar to those in heart. Three additional mouse genes and two human genes closely related to mBCNG-1 display unique patterns of mRNA expression in different tissues, including brain and heart, demonstrating that these channels constitute a widely expressed gene family.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Barium / pharmacology
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Biological Clocks / genetics*
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Brain / physiology*
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Cesium / pharmacology
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Cloning, Molecular
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Cyclic AMP / pharmacology
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Cyclic Nucleotide-Gated Cation Channels
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DNA, Complementary / genetics
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Electric Conductivity
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Gene Expression
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Heart / physiology
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Humans
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Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
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Ion Channels / antagonists & inhibitors
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Ion Channels / biosynthesis
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Ion Channels / genetics*
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Mice
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Molecular Sequence Data
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Multigene Family
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Nerve Tissue Proteins / antagonists & inhibitors
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Nerve Tissue Proteins / biosynthesis
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Nerve Tissue Proteins / genetics*
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Neuroglia / metabolism
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Oocytes
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Pacemaker, Artificial
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Patch-Clamp Techniques
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Potassium / metabolism
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Potassium Channels
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Recombinant Proteins / biosynthesis
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Sequence Homology, Amino Acid
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Sodium / metabolism
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Species Specificity
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Tissue Distribution
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Xenopus
Substances
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Cyclic Nucleotide-Gated Cation Channels
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DNA, Complementary
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Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
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Ion Channels
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Nerve Tissue Proteins
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Potassium Channels
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Recombinant Proteins
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Cesium
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Barium
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Sodium
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Cyclic AMP
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Potassium
Associated data
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GENBANK/AF064873
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GENBANK/AF064874
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GENBANK/AF064875
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GENBANK/AF064876
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GENBANK/AF064877