Molecular cloning and expression of human cGMP-binding cGMP-specific phosphodiesterase (PDE5)

Biochem Biophys Res Commun. 1998 Jun 18;247(2):249-54. doi: 10.1006/bbrc.1998.8769.

Abstract

A human PDE5 cDNA has been isolated which contains an open reading frame encoding an 875 amino acid, 100,012 Da polypeptide, the expression of which yields a protein of the predicted size and is capable of hydrolyzing cGMP. The deduced amino acid sequence is very similar (95%) to that of bovine PDE5, and comprises a conserved cGMP-binding domain and catalytic domain. Northern analysis reveals a major and minor transcript of approximately 9 kb and approximately 8 kb respectively, thus indicating the existence of at least two splice variants, the major form being readily detected in bladder, colon, lung, pancreas, placenta, prostate, small intestine, and stomach.

Publication types

  • Comparative Study

MeSH terms

  • 3',5'-Cyclic-GMP Phosphodiesterases / chemistry
  • 3',5'-Cyclic-GMP Phosphodiesterases / genetics*
  • 3',5'-Cyclic-GMP Phosphodiesterases / metabolism
  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cloning, Molecular
  • Conserved Sequence
  • Cyclic Nucleotide Phosphodiesterases, Type 5
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Female
  • Gene Expression
  • Humans
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Pregnancy
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • DNA Primers
  • DNA, Complementary
  • RNA, Messenger
  • 3',5'-Cyclic-GMP Phosphodiesterases
  • Cyclic Nucleotide Phosphodiesterases, Type 5
  • PDE5A protein, human

Associated data

  • GENBANK/AJ004865