Abstract
The expression and activity of phospholipase A2 (PLA2) isoforms were investigated in primary cultures of striatal astrocytes. The calcium ionophore A23187 together with the protein kinase C activator phorbol ester was the most potent stimulus in eliciting [3H]arachidonic acid release in the extracellular medium. Reverse transcription coupled to polymerase chain reaction (RT-PCR) showed the presence of the 85 kDa cytosolic PLA2 mRNA and the 14 kDa secretory PLA2 mRNA in untreated astrocytes. Immunoblot experiments with isoform-specific antibodies showed the presence of the cytosolic PLA2 in untreated astrocytes, while the secretory PLA2 was detected only in lipopolysaccharide-treated astrocytes. These data suggest that the two PLA2 isoforms expressed in striatal astrocytes might play different roles in cellular processes mediated by astrocytes.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Arachidonic Acid / metabolism
-
Astrocytes / drug effects
-
Astrocytes / enzymology*
-
Calcimycin / pharmacology
-
Corpus Striatum / enzymology*
-
Enzyme Activation / drug effects
-
Enzyme Induction / drug effects
-
Enzyme Inhibitors / pharmacology
-
Ionophores / pharmacology
-
Isoenzymes / biosynthesis*
-
Isoenzymes / genetics
-
Nerve Tissue Proteins / biosynthesis*
-
Nerve Tissue Proteins / genetics
-
Phospholipases A / biosynthesis*
-
Phospholipases A / genetics
-
Phospholipases A2
-
Polymerase Chain Reaction
-
Protein Kinase C / metabolism
-
Quinacrine / pharmacology
-
RNA, Messenger / biosynthesis
-
Rats
-
Tetradecanoylphorbol Acetate / pharmacology
Substances
-
Enzyme Inhibitors
-
Ionophores
-
Isoenzymes
-
Nerve Tissue Proteins
-
RNA, Messenger
-
Arachidonic Acid
-
Calcimycin
-
Protein Kinase C
-
Phospholipases A
-
Phospholipases A2
-
Quinacrine
-
Tetradecanoylphorbol Acetate