Blue native PAGE (BN-PAGE) can be used for one-step isolation of protein complexes from
biological membranes and total cell and tissue homogenates. It can also be used to …

Macromolecular complexes are essential players in numerous biological processes. They
are often large, dynamic, and rather labile; approaches to study them are scarce. Covering …

Clear‐native PAGE (CN‐PAGE) separates acidic water‐soluble and membrane proteins (pI
< 7) in an acrylamide gradient gel, and usually has lower resolution than blue‐native PAGE (…

Leber’s hereditary optic neuropathy (LHON) is the most frequent mitochondrial disease and
was the first to be genetically defined by a point mutation in mitochondrial DNA (mtDNA). A …

Clear native electrophoresis and blue native electrophoresis are microscale techniques for
the isolation of membrane protein complexes. The Coomassie Blue G-250 dye, used in blue …

We used electron cryotomography of mitochondrial membranes from wild-type and mutant
Saccharomyces cerevisiae to investigate the structure and organization of ATP synthase …

Aims: Intracellular amyloid beta (Aβ) oligomers and extracellular Aβ plaques are key
players in the progression of sporadic Alzheimer's disease (AD). Still, the molecular signals …

An isolated defect of respiratory chain complex I activity is a frequent biochemical abnormality
in mitochondrial disorders. Despite intensive investigation in recent years, in most …

Dimerization or oligomerization of ATP synthase has been proposed to play an important
role for mitochondrial cristae formation and to be involved in regulating ATP synthase activity. …

Respiratory complex I (NADH: ubiquinone oxidoreductase) is a large mitochondrial inner
membrane enzyme consisting of 45 subunits and 8 iron-sulfur (Fe/S) clusters. While complex I …