Mechanisms of release of nucleotides and integration of their action as P2X-and P2Y-receptor activating molecules

ER Lazarowski, RC Boucher, TK Harden - Molecular pharmacology, 2003 - ASPET
The concept that adenine and uridine nucleotides function as extracellular signaling molecules
has been markedly expanded in the last decade. At least 15 nucleotide-activated cell …

[HTML][HTML] Towards a revised nomenclature for P1 and P2 receptors

…, G Burnstock, GR Dubyak, TK Harden… - Trends in …, 1997 - ncbi.nlm.nih.gov
The classification of receptors for adenosine, ATP and ADP (collectively called purinoceptors)
has seen a number of developments in the past three years. The important division of …

The phospholipase C isozymes and their regulation

A Gresset, J Sondek, TK Harden - Phosphoinositides I: Enzymes of …, 2012 - Springer
The physiological effects of many extracellular neurotransmitters, hormones, growth factors,
and other stimuli are mediated by receptor-promoted activation of phospholipase C (PLC) …

Nucleotides released by apoptotic cells act as a find-me signal to promote phagocytic clearance

…, M Ostankovich, P Sharma, JJ Lysiak, TK Harden… - Nature, 2009 - nature.com
Phagocytic removal of apoptotic cells occurs efficiently in vivo such that even in tissues with
significant apoptosis, very few apoptotic cells are detectable 1 . This is thought to be due to …

[HTML][HTML] Constitutive release of ATP and evidence for major contribution of ecto-nucleotide pyrophosphatase and nucleoside diphosphokinase to extracellular …

ER Lazarowski, RC Boucher, TK Harden - Journal of Biological Chemistry, 2000 - ASBMB
Nucleotides are important extracellular signaling molecules. At least five mammalian P2Y
receptors exist that are specifically activated by ATP, UTP, ADP, or UDP. Although the …

[HTML][HTML] The experimental power of FR900359 to study Gq-regulated biological processes

…, J Gomeza, HG Dohlman, J Sondek, TK Harden… - Nature …, 2015 - nature.com
Despite the discovery of heterotrimeric αβγ G proteins ∼25 years ago, their selective perturbation
by cell-permeable inhibitors remains a fundamental challenge. Here we report that the …

Kinetic Scaffolding Mediated by a Phospholipase C–β and Gq Signaling Complex

…, X Wang, C Montell, T Kozasa, J Sondek, TK Harden - Science, 2010 - science.org
Transmembrane signals initiated by a broad range of extracellular stimuli converge on
nodes that regulate phospholipase C (PLC)–dependent inositol lipid hydrolysis for signal …

[HTML][HTML] Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation

…, L Homolya, RC Boucher, TK Harden - Journal of Biological …, 1997 - ASBMB
ATP is released from most cell types and functions as an extracellular signaling molecule
through activation of members of the two large families of P2X and P2Y receptors. Although …

Pharmacological selectivity of the cloned human P2U-purinoceptor: potent activation by diadenosine tetraphosphate.

…, MJ Stutts, RC Boucher, TK Harden - British journal of …, 1995 - ncbi.nlm.nih.gov
The human P2U-purinoceptor was stably expressed in 1321N1 human astrocytoma cells and
the pharmacological selectivity of the expressed receptor was studied by measurement of …

[HTML][HTML] Beta gamma-subunit activation of G-protein-regulated phospholipase C.

JL Boyer, GL Waldo, TK Harden - Journal of Biological Chemistry, 1992 - Elsevier
The availability of purified G alpha 11 and the G-protein-regulated phospholipase C from
turkey erythrocytes has allowed an examination of the direct effects of G-protein beta gamma-…