Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Pharmacological Reviews
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Pharmacological Reviews

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit Pharm Rev on Facebook
  • Follow Pharm Rev on Twitter
  • Follow ASPET on LinkedIn
Review ArticleReview Article

Mass Spectrometry Approaches Empowering Neuropeptide Discovery and Therapeutics

Krishna D. B. Anapindi, Elena V. Romanova, James W. Checco and Jonathan V. Sweedler
Vivian Hook, ASSOCIATE EDITOR
Pharmacological Reviews July 2022, 74 (3) 662-679; DOI: https://doi.org/10.1124/pharmrev.121.000423
Krishna D. B. Anapindi
Department of Chemistry and the Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, Illinois (K.D.B.A., E.V.R., J.V.S.) and Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska (J.W.C.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elena V. Romanova
Department of Chemistry and the Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, Illinois (K.D.B.A., E.V.R., J.V.S.) and Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska (J.W.C.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
James W. Checco
Department of Chemistry and the Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, Illinois (K.D.B.A., E.V.R., J.V.S.) and Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska (J.W.C.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan V. Sweedler
Department of Chemistry and the Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, Illinois (K.D.B.A., E.V.R., J.V.S.) and Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska (J.W.C.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vivian Hook
Roles: ASSOCIATE EDITOR
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Figure
    • Download figure
    • Open in new tab
    • Download powerpoint
  • Fig. 1
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 1

    Peptides are generated from larger precursor proteins in multiple steps: pre-propeptide synthesis in endoplasmic reticulum, packaging into dense-core secretory vesicles in the Golgi apparatus with proteolytic processing in the vesicles, transport to release sites, and finally, the stimulus-dependent secretion of mature bioactive peptides.

  • Fig. 2
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 2

    The analytical framework for neuropeptide characterization highlighting the major steps of sample preparation, MS-based measurement, and informatics, which combine to provide an exceptional toolset for neuropeptide discovery.

  • Fig. 3
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 3

    PTM characterization via MS and their functional implications. MS is effective at discovering both common and unexpected chemical modifications to peptides; these modifications impact receptor binding and lifetime and, hence, need to be characterized to test a peptide’s function.

PreviousNext
Back to top

In this issue

Pharmacological Reviews: 74 (3)
Pharmacological Reviews
Vol. 74, Issue 3
1 Jul 2022
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Pharmacological Reviews article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Mass Spectrometry Approaches Empowering Neuropeptide Discovery and Therapeutics
(Your Name) has forwarded a page to you from Pharmacological Reviews
(Your Name) thought you would be interested in this article in Pharmacological Reviews.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Review ArticleReview Article

Mass Spectrometry for Neuropeptide Discovery

Krishna D. B. Anapindi, Elena V. Romanova, James W. Checco and Jonathan V. Sweedler
Pharmacological Reviews July 1, 2022, 74 (3) 662-679; DOI: https://doi.org/10.1124/pharmrev.121.000423

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Review ArticleReview Article

Mass Spectrometry for Neuropeptide Discovery

Krishna D. B. Anapindi, Elena V. Romanova, James W. Checco and Jonathan V. Sweedler
Pharmacological Reviews July 1, 2022, 74 (3) 662-679; DOI: https://doi.org/10.1124/pharmrev.121.000423
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Visual Overview
    • Abstract
    • I. Introduction: A Century of Neuropeptide/Hormone Discovery: Where Are We and Where Are We Going?
    • II. What Are Neuropeptides and Peptide Hormones?
    • III. Methods of Neuropeptide Discovery and Characterization
    • IV. From Discovery to Function
    • V. Unusual Routes to Peptide Formation and Function
    • VI. The Rise of Biologics: Therapeutic Peptides
    • VII. Conclusions
    • Authorship Contributions
    • Footnotes
    • Abbreviations
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • PROKINETICIN RECEPTORS AS THERAPEUTIC TARGETS
  • ASO and siRNA drugs for systemic diseases of liver origin
  • β-Arrestins: Structure, Function, and Physiology
Show more Review article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Pharmacological Reviews
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Molecular Pharmacology
  • Pharmacology Research & Perspectives
ISSN 1521-0081 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics